Article
Rapid detection and quantitation of BRAF mutations in hairy cell leukemia using a sensitive pyrosequencing assay.
American journal of clinical pathology - 1 Jul 2012
Verma Shalini, Greaves Wesley O, Ravandi Farhad, Reddy Neelima, Bueso-Ramos Carlos E, O'Brien Susan, Thomas Deborah A, Kantarjian Hagop, Medeiros L Jeffrey, Luthra Rajyalakshmi, Patel Keyur P
Abstract excerpt
BRAF protooncogene is an important mediator of cell proliferation and survival signals. BRAF p.V600E mutation was recently described as a molecular marker of hairy cell leukemia (HCL). We developed and validated a pyrosequencing-based approach that covers BRAF mutational hotspots in exons 11 (codon 468) and 15 (codons 595 to 600). The assay detects BRAF mutations at an analytical sensitivity of 5%. We screened 16...
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