Purification of three related peripheral membrane proteins needed for vesicular transport.

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Article

Purification of three related peripheral membrane proteins needed for vesicular transport.

The Journal of biological chemistry - 15 Jun 1990

Clary D O, Rothman J E

Abstract excerpt

We report conditions under which Golgi membranes depleted of peripheral membrane proteins can be reconstituted for intra-cisternal vesicular transport. Analysis of the reconstitution reveals requirements for N-ethylmaleimide-sensitive fusion protein, a purified peripheral protein involved in the...

Topics

Animals
Biological Transport
Carrier Proteins
Cell Line
Chromatography, DEAE-Cellulose
Chromatography, Gel
Chromatography, Ion Exchange
Cytosol
Electrophoresis, Polyacrylamide Gel
Golgi Apparatus
Intracellular Membranes
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Membrane Proteins

Molecular Weight

Mutation

N-Ethylmaleimide-Sensitive Proteins

Peptide Mapping

Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 2190980

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2190980-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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