Article
In vitro reconstitution of intercompartmental protein transport to the yeast vacuole.
The Journal of cell biology - 1 Dec 1990
Vida T A, Graham T R, Emr S D
Abstract excerpt
Toward a detailed understanding of protein sorting in the late secretory pathway, we have reconstituted intercompartmental transfer and proteolytic maturation of a yeast vacuolar protease, carboxypeptidase Y (CPY). This in vitro reconstitution uses permeabilized yeast spheroplasts that are first...
Topics
- Adenosine Triphosphate
- Biological Transport
- Carboxypeptidases
- Cathepsin A
- Cell Fractionation
- Cell-Free System
- Cytoplasm
- Fungal Proteins
- Intracellular Membranes
- Mutation
- Protein Processing, Post-Translational
- Protoplasts
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Vacuoles