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Article

In vitro reconstitution of intercompartmental protein transport to the yeast vacuole.

The Journal of cell biology - 1 Dec 1990

Vida T A, Graham T R, Emr S D

Abstract excerpt

Toward a detailed understanding of protein sorting in the late secretory pathway, we have reconstituted intercompartmental transfer and proteolytic maturation of a yeast vacuolar protease, carboxypeptidase Y (CPY). This in vitro reconstitution uses permeabilized yeast spheroplasts that are first radiolabeled in vivo under conditions that kinetically trap ER and Golgi apparatus-modified precursor forms of CPY (p1...

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Identifiers

PMID: 2269659
DOI: 10.1083/jcb.111.6.2871
PMCID: PMC2116412

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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