Immunoisolation of Kex2p-containing organelles from yeast demonstrates colocalisation of three processing proteinases to a single Golgi compartment.

Abstract excerpt

One of the Golgi compartments of Saccharomyces cerevisiae is defined by the presence of a specific endoproteinase, Kex2p, which cleaves precursor polypeptides at pairs of basic residues. We have used antibodies directed against the cytoplasmically disposed C-terminal domain of Kex2p to develop an immuno-affinity procedure for the isolation of Kex2p-containing organelles. The method gives a high yield of sealed...

Topics

• Carboxypeptidases
• Centrifugation, Density Gradient
• Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
• Golgi Apparatus
• Immunoblotting
• Immunohistochemistry
• Mutation
• Organelles
• Proprotein Convertases
• Protein Processing, Post-Translational
• Saccharomyces cerevisiae
• Saccharomyces cerevisiae Proteins
• Subtilisins