Article
Use of sec mutants to define intermediates in protein transport from endoplasmic reticulum.
Methods in enzymology - 1 Jan 1992
Rexach M F, Schekman R W
Abstract excerpt
In this chapter we have discussed the methodology used to identify and characterize three intermediates in protein transport from the ER that represent stages of transport vesicle budding, targeting, and fusion. The intermediates are obtained using a variety of transport inhibitors: low-temperature incubations, addition of chemicals, or inactivation of Sec protein function using temperature-sensitive mutants or...
Topics
- Biological Transport
- Calcium
- Cell Compartmentation
- Cell Membrane Permeability
- Cold Temperature
- Endoplasmic Reticulum
- Golgi Apparatus
- Kinetics
- Mating Factor
- Membrane Fusion
- Mutation