Article
Functional telomere formation in yeast using synthetic C4A2 sequences.
Plasmid - 1 Jan 1990
Ascenzioni F, Guerrini A M, Donini P
Abstract excerpt
A yeast artificial chromosome (YAC) was constructed with a native autonomous replicating sequence (ARS) flanked telomere at one end and a 50-bp synthetic oligonucleotide of C4A2 repeats at the other. This was done in order to determine whether the presence of the flanking ARS sequence is required...
Topics
- Adenosine Triphosphate
- Base Sequence
- Chromosomes, Fungal
- DNA Replication
- DNA, Fungal
- Genes, Fungal
- Mitosis
- Mutation
- Plasmids
- Repetitive Sequences, Nucleic Acid
- Restriction Mapping
- Saccharomyces cerevisiae
- Transformation, Genetic