Article
Detection of homologous recombination between yeast artificial chromosomes with overlapping inserts.
Nucleic acids research - 11 Mar 1991
Cellini A, Lacatena R M, Tocchini-Valentini G P
Abstract excerpt
We have developed a system which facilitates the detection of recombination between Yeast Artificial Chromosomes (YAC's) carrying homologous inserts. The system consists of a classical YAC vector, a new YAC vector and two appropriately labelled yeast strains of opposite mating type. The new YAC vector differs in markers from the canonical YAC vector. To test whether homologous recombination takes place, phage...
Topics
- Bacteriophage lambda
- Chromosomes, Fungal
- Cloning, Molecular
- Genetic Vectors
- Meiosis
- Phenotype
- Recombination, Genetic
- Sequence Homology, Nucleic Acid