Article
Functional reconstitution of wild-type and mutant Tetrahymena telomerase.
Genes & development - 1 Mar 1994
Autexier C, Greider C W
Abstract excerpt
Telomerase is a ribonucleoprotein that catalyzes telomere elongation in vitro and in vivo. The 159-nucleotide RNA component of Tetrahymena telomerase contains the sequence 5'-CAACCCCAA-3' ("template region"), which serves as a template for the addition of the sequence d(TTGGGG)n to Tetrahymena telomeres. To dissect the Tetrahymena telomerase enzyme mechanism, we developed a functional in vitro reconstitution...
Topics
- Animals
- Base Sequence
- DNA Nucleotidylexotransferase
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- RNA
- Repetitive Sequences, Nucleic Acid
- Telomere
- Templates, Genetic
- Tetrahymena