Article
An approach to crystallizing proteins by metal-mediated synthetic symmetrization.
Protein science : a publication of the Protein Society - 1 Nov 2011
Laganowsky Arthur, Zhao Minglei, Soriaga Angela B, Sawaya Michael R, Cascio Duilio, Yeates Todd O
Abstract excerpt
Combining the concepts of synthetic symmetrization with the approach of engineering metal-binding sites, we have developed a new crystallization methodology termed metal-mediated synthetic symmetrization. In this method, pairs of histidine or cysteine mutations are introduced on the surface of target proteins, generating crystal lattice contacts or oligomeric assemblies upon coordination with metal....
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