Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Metal ions-binding T4 lysozyme as an intramolecular protein purification tag compatible with X-ray crystallography.

Protein science : a publication of the Protein Society - 1 Jun 2017

Boura Evzen, Baumlova Adriana, Chalupska Dominika, Dubankova Anna, Klima Martin

Abstract excerpt

Phage T4 lysozyme is a well folded and highly soluble protein that is widely used as an insertion tag to improve solubility and crystallization properties of poorly behaved recombinant proteins. It has been used in the fusion protein strategy to facilitate crystallization of various proteins including multiple G protein-coupled receptors, lipid kinases, or sterol binding proteins. Here, we present a structural...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteriophage T4
Share to Chromatography, Affinity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Crystallography, X-Ray

Share to Muramidase

Share to Mutation

Source

Open in PubMed Open full text

Identifiers

PMID: 28342173
DOI: 10.1002/pro.3162
PMCID: PMC5441413

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Metal ions-binding T4 lysozyme as an intramolecular protein purification tag compatible with X-ray crystallography.PMID 28342173Read ↗

Expression and purification of a recombinant metal-binding T4 lysozyme fusion protein.Semantic proximity Read ↗

N-terminal T4 lysozyme fusion facilitates crystallization of a G protein coupled receptor.Semantic proximity Read ↗

An approach to crystallizing proteins by metal-mediated synthetic symmetrization.Semantic proximity Read ↗

Strong and heterogeneous adsorption of infectious bursal disease VP2 subviral particle with immobilized metal ions dependent on two surface histidine residues.Semantic proximity Read ↗

Ni(II)-binding affinity of CcNikZ-II and its homologs: the role of the HH-prong and variable loop revealed by structural and mutational studies.Semantic proximity Read ↗

Lessons from the lysozyme of phage T4.Semantic proximity Read ↗

A protocol for cloning, expression, and purification of Lysine exporter (LysE) gene of Mycobacterium tuberculosisSemantic proximity Read ↗

Characterization of the E26H Mutant Schistosoma japonicum Glutathione S-Transferase.Semantic proximity Read ↗

Structural characterization of the Cu(II)-NTA spin label on α-helices by X-ray crystallography and electron paramagnetic resonance.Semantic proximity Read ↗

Using T4 genetics and Laemmli's development of high-resolution SDS gel electrophoresis to reveal structural protein interactions controlling protein folding and phage self-assembly.Semantic proximity Read ↗

In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.Semantic proximity Read ↗

The molecular modification, expression, and the antibacterial effects studies of human lysozyme.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.