Article
An approach to crystallizing proteins by synthetic symmetrization.
Proceedings of the National Academy of Sciences of the United States of America - 31 Oct 2006
Banatao D Rey, Cascio Duilio, Crowley Christopher S, Fleissner Mark R, Tienson Heather L, Yeates Todd O
Abstract excerpt
Previous studies of symmetry preferences in protein crystals suggest that symmetric proteins, such as homodimers, might crystallize more readily on average than asymmetric, monomeric proteins. Proteins that are naturally monomeric can be made homodimeric artificially by forming disulfide bonds between individual cysteine residues introduced by mutagenesis. Furthermore, by creating a variety of single-cysteine...
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