Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.

doi:10.1073/pnas.1108460108

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Article

Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.

Proceedings of the National Academy of Sciences of the United States of America - 20 Sept 2011

Smirnov Ivan, Carletti Eugénie, Kurkova Inna, Nachon Florian, Nicolet Yvain, Mitkevich Vladimir A, Débat Hélène, Avalle Bérangère, Belogurov Alexey A, Kuznetsov Nikita, Reshetnyak Andrey, Masson Patrick, Tonevitsky Alexander G, Ponomarenko Natalia, Makarov Alexander A, Friboulet Alain, Tramontano Alfonso, Gabibov Alexander

Abstract excerpt

Igs offer a versatile template for combinatorial and rational design approaches to the de novo creation of catalytically active proteins. We have used a covalent capture selection strategy to identify biocatalysts from within a human semisynthetic antibody variable fragment library that uses a nucleophilic mechanism. Specific phosphonylation at a single tyrosine within the variable light-chain framework was...

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PMID: 21896761
DOI: 10.1073/pnas.1108460108
PMCID: PMC3179052

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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