Article
[Evaluation of methods for detection of NPM1 gene mutations in acute myeloid leukemia].
Zhongguo shi yan xue ye xue za zhi - 1 Aug 2011
Li Zhi-Peng, Zhang Xuan, Zhao Xiao-Ming, Li Qing-Ge, Chen Ya-Mei, Lu Quan-Yi
Abstract excerpt
The purpose of this study was to establish real-time based methods for detection of NPM1 gene mutation in acute myeloid leukemia (AML). Primers/probes were designed according to the clustered region of NPM1 mutations on exon 12. Two real-time PCR assays, including high resolution melting curve (HRM) and allele-specific PCR (AS-PCR), were developed and clinically evaluated with 89 AML samples, which were...
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