Article
Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli.
Journal of bacteriology - 1 May 1990
Paulus T J, Tuan J S, Luebke V E, Maine G T, DeWitt J P, Katz L
Abstract excerpt
A mutant strain derived by chemical mutagenesis of Saccharopolyspora erythraea (formerly known as Streptomyces erythreus) was isolated that accumulated erythromycin C and, to a lesser extent, its precursor, erythromycin D, with little or no production of erythromycin A or erythromycin B (the 3"-O...
Topics
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- DNA, Bacterial
- Erythromycin
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Methyltransferases
- Mutation
- Plasmids
- Recombinant Proteins
- Restriction Mapping
- Streptomyces