Article
Cloning of genes involved in erythromycin biosynthesis from Saccharopolyspora erythraea using a novel actinomycete-Escherichia coli cosmid.
Gene - 31 May 1990
Tuan J S, Weber J M, Staver M J, Leung J O, Donadio S, Katz L
Abstract excerpt
Two plasmids were constructed that replicate in Saccharopolyspora (Sac.) erythraea, Escherichia coli and Streptomyces (S.) lividans, and used for the cloning of a locus involved in the synthesis of the macrolide antibiotic erythromycin (Er). Plasmid pAL7002 contains the thiostrepton-resistance gene (tsr), a replicon-containing fragment from pJVI and pUC9. Plasmid pNJI contains the lambda cos site but is otherwise...
Topics
- Actinomycetaceae
- Alleles
- Blotting, Southern
- Cloning, Molecular
- Cosmids
- DNA Mutational Analysis
- DNA, Fungal
- Erythromycin
- Escherichia coli
- Genes, Fungal
- Genetic Vectors