Factor Xa cleavage of fusion proteins. Elimination of non-specific cleavage by reversible acylation.

doi:10.1016/0014-5793(90)80696-g

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Article

Factor Xa cleavage of fusion proteins. Elimination of non-specific cleavage by reversible acylation.

FEBS letters - 9 Apr 1990

Wearne S J

Abstract excerpt

A method is described for the elimination of non-specific cleavage of fusion proteins by factor Xa. Putative non-specific cleavage sites C-terminal to lysyl residues are blocked by reversible acylation by 3,4,5,6-tetrahydrophthalic anhydride prior to cleavage. After cleavage, the acyl groups are...

Topics

Acylation
Animals
Cloning, Molecular
Escherichia coli
Factor Xa
Hydrolysis
Molecular Weight
Mutation
Myoglobin
Recombinant Fusion Proteins
Swine

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