Article
Purification and characterization of a mutant human platelet phospholipase A2 expressed in Escherichia coli. Cleavage of a fusion protein with cyanogen bromide.
European journal of biochemistry - 15 Jan 1992
Franken P A, Van den Berg L, Huang J, Gunyuzlu P, Lugtigheid R B, Verheij H M, De Haas G H
Abstract excerpt
Both methionine residues in phospholipase A2 (PLA2) from porcine pancreas have been replaced by leucines with retention of full enzymatic activity. The methionine-less mutant has been expressed as a Cro-LacZ fusion protein in Escherichia coli, from which a pro-PLA2 was liberated by chemical cleav...
Topics
- Acetophenones
- Animals
- Blood Platelets
- Blotting, Western
- Calcium
- Chromatography, Gel
- Crotalid Venoms
- Cyanogen Bromide
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Humans
- Hydrogen-Ion Concentration
- Kinetics
- Micelles