Article
Increased biological activity of a recombinant factor IX variant carrying alanine at position +1.
Protein engineering - 1 Jul 1990
Meulien P, Balland A, Lepage P, Mischler F, Dott K, Hauss C, Grandgeorge M, Lecocq J P
Abstract excerpt
In attempts to improve the post-translational modification and processing of recombinant factor IX (FIX) we have altered the cDNA sequence encoding pre-pro-FIX using site-directed mutagenesis and have expressed the variant cDNAs in BHK21 cells using a vaccinia-virus-derived vector. We find that substitution of the tyrosine residue at +1 for an alanine increases the biological activity of the recombinant molecules...
Topics
- Alanine
- Amino Acid Sequence
- Animals
- Cells, Cultured
- Chromosome Deletion
- Cricetinae
- DNA
- Factor IX
- Gene Expression
- Genetic Variation
- Molecular Sequence Data