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Article

Increased biological activity of a recombinant factor IX variant carrying alanine at position +1.

Protein engineering - 1 Jul 1990

Meulien P, Balland A, Lepage P, Mischler F, Dott K, Hauss C, Grandgeorge M, Lecocq J P

Abstract excerpt

In attempts to improve the post-translational modification and processing of recombinant factor IX (FIX) we have altered the cDNA sequence encoding pre-pro-FIX using site-directed mutagenesis and have expressed the variant cDNAs in BHK21 cells using a vaccinia-virus-derived vector. We find that substitution of the tyrosine residue at +1 for an alanine increases the biological activity of the recombinant molecules...

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Identifiers

PMID: 2217136
DOI: 10.1093/protein/3.7.629

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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