Article
Proteolytic cleavage of wild type and mutants of the F protein of human parainfluenza virus type 3 by two subtilisin-like endoproteases, furin and Kex2.
Journal of virology - 1 Apr 1994
Ortmann D, Ohuchi M, Angliker H, Shaw E, Garten W, Klenk H D
Abstract excerpt
The fusion (F) protein of human parainfluenza virus type 3 contains the tribasic cleavage site R-T-K-R, which was altered by site-directed mutagenesis. Wild-type F protein and various mutants were expressed by recombinant vaccinia viruses. The endogenous endoprotease present in CV-1 cells cleaves...
Topics
- Amino Acid Sequence
- Base Sequence
- Furin
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Parainfluenza Virus 3, Human
- Proprotein Convertases
- Recombinant Proteins
- Saccharomyces cerevisiae Proteins
- Subtilisins
- Vaccinia virus
- Viral Fusion Proteins