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Article

Strategies for optimization of short-term genotoxicity tests: the synergistic effect of NADPH and NADH on P450 function in processing pre-mutagens.

Mutagenesis - 1 Jan 1990

Paolini M, Sapigni E, Hrelia P, Grilli S, Lattanzi G, Scotti M, Cantelli Forti G

Abstract excerpt

The synergistic effect of NADPH and NADH on P450 functions upon pre-mutagens requiring metabolism during the incubation conditions used in the liver microsomal assay (LMA) was studied. The mean specific activity (Asp) during 1 h of pre-incubation (LMA) of some microsomal mono-oxygenases (i.e. ethylmorphine N-demethylase, p-nitroanisole O-demethylase and aminopyrine N-demethylase) examined with S9 fractions from...

Topics

Animals
Cytochrome P-450 Enzyme System
Drug Synergism
Enzyme Activation
Ethane
Hydrocarbons, Chlorinated
Lipid Peroxidation
Male
Mice
Microsomes, Liver
Mutagenicity Tests

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

NAD

NADP

Oxidoreductases

Saccharomyces cerevisiae

Source

Identifiers

PMID: 2184312
DOI: 10.1093/mutage/5.1.51

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2184312-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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