Article
Strategies for advancement of short-term mutagenicity tests: on the optimal ionic strength for the liver microsomal assay.
Carcinogenesis - 1 Aug 1992
Paolini M, Mesirca R, Pozzetti L, Silingardi P, Grilli S, Cantelli-Forti G
Abstract excerpt
The aim of this work was to optimize the ionic strength (tau) in the liver microsomal assay (LMA) in performing short-term genotoxicity tests. tau optimization would increase the sensitivity (i.e. decrease false negatives) and at the same time increase the specificity (decrease false positives). Such optimization depends upon the relative activities and stabilities of the liver polysubstrate cytochrome P450- and...
Topics
- Animals
- DNA
- Female
- In Vitro Techniques
- Lipid Peroxidation
- Male
- Mice
- Microsomes, Liver
- Mutagenicity Tests
- Mutation
- Osmolar Concentration
- Sensitivity and Specificity