Article
DnaK heat shock protein of Escherichia coli maintains the negative supercoiling of DNA against thermal stress.
The Journal of biological chemistry - 15 Nov 1996
Ogata Y, Mizushima T, Kataoka K, Kita K, Miki T, Sekimizu K
Abstract excerpt
Plasmid DNA in exponentially growing Escherichia coli immediately relaxes after heat shock, and the relaxed state of DNA rapidly reverts to the original state with exposure to conditions of heat shock. We have now obtained genetic and biochemical evidence indicating that DnaK heat shock protein of E. coli, a prokaryotic homologue of hsp70, is involved in this re-supercoiling of DNA. As re-supercoiling of DNA did...
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