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Article

Site-directed mutagenesis of arginine 179 of thymidylate synthase. A nonessential substrate-binding residue.

The Journal of biological chemistry - 25 Apr 1990

Santi D V, Pinter K, Kealey J, Davisson V J

Abstract excerpt

X-ray structural studies have shown that Arg-179 of thymidylate synthase is complexed to bound inorganic phosphate or to the 5'-phosphate of the bound substrate dUMP. The importance of Arg-179 to the structure/function of thymidylate synthase is also indicated by its complete conservation among the 17 thymidylate synthases thus far sequenced. In the present work, Arg-179 has been replaced by Thr, Ala, Lys, and...

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PMID: 2182628

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Site-directed mutagenesis of arginine 179 of thymidylate synthase. A nonessential substrate-binding residue.PMID 2182628Read ↗

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