Article
Site-directed mutagenesis of arginine 179 of thymidylate synthase. A nonessential substrate-binding residue.
The Journal of biological chemistry - 25 Apr 1990
Santi D V, Pinter K, Kealey J, Davisson V J
Abstract excerpt
X-ray structural studies have shown that Arg-179 of thymidylate synthase is complexed to bound inorganic phosphate or to the 5'-phosphate of the bound substrate dUMP. The importance of Arg-179 to the structure/function of thymidylate synthase is also indicated by its complete conservation among the 17 thymidylate synthases thus far sequenced. In the present work, Arg-179 has been replaced by Thr, Ala, Lys, and...
Topics
- Amino Acid Sequence
- Arginine
- Base Sequence
- Binding Sites
- DNA, Single-Stranded
- Escherichia coli
- Genetic Vectors
- Kinetics
- Molecular Sequence Data
- Mutation
- Plasmids