Exclusion of 2'-deoxycytidine 5'-monophosphate by asparagine 229 of thymidylate synthase.

doi:10.1021/bi00087a001

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Article

Exclusion of 2'-deoxycytidine 5'-monophosphate by asparagine 229 of thymidylate synthase.

Biochemistry - 14 Sept 1993

Liu L, Santi D V

Abstract excerpt

In thymidylate synthase (TS, EC 2.1.1.45), the only side chain in direct hydrogen bonding with the pyrimidine ring of the substrate dUMP is asparagine 229 (N229). In binary and ternary complexes, the carboxamide moiety of the side chain of N229 forms a cyclic hydrogen bond network bridging N-3 an...

Topics

Asparagine
Deoxycytidine Monophosphate
Hydrogen Bonding
Kinetics
Mutation
Spectrophotometry, Ultraviolet
Thymidylate Synthase

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