Article
Replacement of Val3 in human thymidylate synthase affects its kinetic properties and intracellular stability .
Biochemistry - 23 Mar 2010
Huang Xiao, Gibson Lydia M, Bell Brittnaie J, Lovelace Leslie L, Peña Maria Marjorette O, Berger Franklin G, Berger Sondra H, Lebioda Lukasz
Abstract excerpt
Human and other mammalian thymidylate synthase (TS) enzymes have an N-terminal extension of approximately 27 amino acids that is not present in bacterial TSs. The extension, which is disordered in all reported crystal structures of TSs, has been considered to play a primary role in protein turnover but not in catalytic activity. In mammalian cells, the variant V3A has a half-life similar to that of wild-type...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.