Article
Delineation of the roles of amino acids involved in the catalytic functions of Leuconostoc mesenteroides glucose 6-phosphate dehydrogenase.
Biochemistry - 12 Dec 2000
Vought V, Ciccone T, Davino M H, Fairbairn L, Lin Y, Cosgrove M S, Adams M J, Levy H R
Abstract excerpt
The roles of particular amino acids in substrate and coenzyme binding and catalysis of glucose-6-phosphate dehydrogenase of Leuconostoc mesenteroides have been investigated by site-directed mutagenesis, kinetic analysis, and determination of binding constants. The enzyme from this species has functional dual NADP(+)/NAD(+) specificity. Previous investigations in our laboratories determined the three-dimensional...
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