Article
Site-directed mutagenesis of mouse thymidylate synthase: alteration of Arg44 to Val44 in a conserved loop guarding the active site has striking effects on catalysis and nucleotide binding.
Biochemical and biophysical research communications - 30 Mar 1990
Zhang H C, Cisneros R J, Deng W L, Johnson L F, Dunlap R B
Abstract excerpt
The arginine located at position 44 of mouse thymidylate synthase is in a highly conserved loop that is in close proximity to the active site cleft of the enzyme. Structural analyses have suggested that this arginine forms hydrogen bonds with the alpha-carboxylate of the C-terminal amino acid and the phosphate of the substrate analog, FdUMP (D. A. Matthews, K. Appelt and S. J. Oatley, (1989) Adv. Enz. Reg., 29,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.