Article
Site-directed mutagenesis of mouse thymidylate synthase: alteration of Arg44 to Val44 in a conserved loop guarding the active site has striking effects on catalysis and nucleotide binding.
Biochemical and biophysical research communications - 30 Mar 1990
Zhang H C, Cisneros R J, Deng W L, Johnson L F, Dunlap R B
Abstract excerpt
The arginine located at position 44 of mouse thymidylate synthase is in a highly conserved loop that is in close proximity to the active site cleft of the enzyme. Structural analyses have suggested that this arginine forms hydrogen bonds with the alpha-carboxylate of the C-terminal amino acid and the phosphate of the substrate analog, FdUMP (D. A. Matthews, K. Appelt and S. J. Oatley, (1989) Adv. Enz. Reg., 29,...
Topics
- Amino Acid Sequence
- Animals
- Arginine
- Base Sequence
- Binding Sites
- Catalysis
- Deoxyuracil Nucleotides
- Fluorodeoxyuridylate
- Hydrogen Bonding
- Kinetics