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Article

Distribution of mutations in human thymidylate synthase yielding resistance to 5-fluorodeoxyuridine.

The Journal of biological chemistry - 27 Sept 2002

Kawate Hisaya, Landis Daniel M, Loeb Lawrence A

Abstract excerpt

Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and is the target of cancer chemotherapeutic agents (e.g. 5-fluorouracil). Here, we used error-prone PCR to mutagenize the full-length human TS cDNA and then selected mutants resistant to 5-fluorodeoxyuridine in a bacterial complementation system. We found that resistant mutants contained 1-5 amino acid substitutions and that these substitutions were...

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Source

Identifiers

PMID: 12147691
DOI: 10.1074/jbc.M204956200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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