Article
Mutation of asparagine 229 to aspartate in thymidylate synthase converts the enzyme to a deoxycytidylate methylase.
Biochemistry - 9 Jun 1992
Liu L, Santi D V
Abstract excerpt
The conserved Asn 229 of thymidylate synthase (TS) forms a cyclic hydrogen bond network with the 3-NH and 4-O of the nucleotide substrate dUMP. The Asn 229 to Asp mutant of Lactobacillus casei thymidylate synthase (TS N229D) has been prepared, purified, and investigated. Steady-state kinetic para...
Topics
- Amino Acid Sequence
- Asparagine
- Aspartic Acid
- Base Sequence
- Catalysis
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Kinetics
- Lacticaseibacillus casei
- Molecular Sequence Data
- Mutation
- Sequence Alignment
- Spectrophotometry, Ultraviolet
- Substrate Specificity
- Thymidylate Synthase