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Article

Crystal structures of a marginally active thymidylate synthase mutant, Arg 126-->Glu.

Protein science : a publication of the Protein Society - 1 Dec 1997

Strop P, Changchien L, Maley F, Montfort W R

Abstract excerpt

Thymidylate synthase (TS) is a long-standing target for anticancer drugs and is of interest for its rich mechanistic features. The enzyme catalyzes the conversion of dUMP to dTMP using the co-enzyme methylenetetrahydrofolate, and is perhaps the best studied of enzymes that catalyze carbon-carbon bond formation. Arg 126 is found in all TSs but forms only 1 of 13 hydrogen bonds to dUMP during catalysis, and just...

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PMID: 9416600
DOI: 10.1002/pro.5560061203
PMCID: PMC2143623

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