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Article

Partitioning roles of side chains in affinity, orientation, and catalysis with structures for mutant complexes: asparagine-229 in thymidylate synthase.

Biochemistry - 23 Apr 1996

Finer-Moore J S, Liu L, Schafmeister C E, Birdsall D L, Mau T, Santi D V, Stroud R M

Abstract excerpt

Thymidylate synthase (TS) methylates only dUMP, not dCMP. The crystal structure of TS.dCMP shows sCMP 4-NH2 excluded from the space between Asn-229 and His-199 by the hydrogen bonding and steric properties and Asn-229. Consequently, 6-C of dCMP is over 4 A from the active site sulfhydryl. The Asn-229 side chain is prevented from flipping 180 degrees to and orientation the could hydrogen bond to dCMP by a hydrogen...

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PMID: 8611496
DOI: 10.1021/bi952751x

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