Article
The three operators of the lac operon cooperate in repression.
The EMBO journal - 1 Apr 1990
Oehler S, Eismann E R, Krämer H, Müller-Hill B
Abstract excerpt
We tested the effect of systematic destruction of all three lac operators of the chromosomal lac operon of Escherichia coli on repression by Lac repressor. Absence of just one 'pseudo-operator' O2 or O3 decreases repression by wild-type tetrameric Lac repressor approximately 2- to 3-fold; absence of both 'pseudo-operators' decreases repression greater than 50-fold. O1 alone represses under these conditions only...
Topics
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Genotype
- Lac Operon
- Macromolecular Substances
- Molecular Sequence Data
- Oligonucleotide Probes
- Plasmids
- Repressor Proteins
- Transcription Factors