Article
Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes.
Applied and environmental microbiology - 1 Sept 2011
Feng Peter C H, Jinneman Karen, Scheutz Flemming, Monday Steven R
Abstract excerpt
Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.
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