Article
Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis.
Applied and environmental microbiology - 1 Oct 2004
De Baets Liesbet, Van der Taelen Imme, De Filette Marina, Piérard Denis, Allison Lesley, De Greve Henri, Hernalsteens Jean-Pierre, Imberechts Hein
Abstract excerpt
Shiga toxins Stx1 and Stx2 play a prominent role in the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections. Several variants of the stx(2) gene, encoding Stx2, have been described. In this study, we developed a PCR-restriction fragment length polymorphism system for typing stx(2) genes of STEC strains. The typing system discriminates eight described variants and allows the identification of...
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