Article
Relationship between stx genotype and Stx2 expression level in Shiga toxin-producing Escherichia coli O157 strains.
Japanese journal of infectious diseases - 1 Jul 2012
Kawano Kimiko, Ono Hidetoshi, Iwashita Osamu, Kurogi Mai, Haga Takeshi, Maeda Ken, Goto Yoshitaka
Abstract excerpt
To determine the expression level of Shiga toxin (Stx) 2-related toxins (Stx2 and Stx2c) produced by each of 33 Stx-producing Escherichia coli (STEC) O157 strains, stx2 and stx2c mRNAs (stx2-related mRNA) were measured using real-time PCR with primers that recognize sequences common to stx2 and stx2c. The amount of Stx2 and Stx2c protein was measured using a reversed passive latex agglutination (RPLA) kit....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
