Article
Inconsistent PCR detection of Shiga toxin-producing Escherichia coli: Insights from whole genome sequence analyses.
PloS one - 1 Jan 2021
Castro Vinicius Silva, Ortega Polo Rodrigo, Figueiredo Eduardo Eustáquio de Souza, Bumunange Emmanuel Wihkochombom, McAllister Tim, King Robin, Conte-Junior Carlos Adam, Stanford Kim
Abstract excerpt
Shiga toxin-producing Escherichia coli (STEC) have been linked to food-borne disease outbreaks. As PCR is routinely used to screen foods for STEC, it is important that factors leading to inconsistent detection of STEC by PCR are understood. This study used whole genome sequencing (WGS) to investigate causes of inconsistent PCR detection of stx1, stx2, and serogroup-specific genes. Fifty strains isolated from...
Topics
- Bacteriophages
- Base Sequence
- Biofilms
- Multilocus Sequence Typing
- Phylogeny
- Plasmids
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Serogroup
- Shiga-Toxigenic Escherichia coli