Detection and Characterization of Shiga Toxigenic <i>Escherichia coli</i> by Using Multiplex PCR Assays for <i>stx</i> ₁ , <i>stx</i> ₂ , <i>eaeA</i> , Enterohemorrhagic <i>E. coli hlyA</i> , <i>rfb</i> _O111 , and <i>rfb</i> _O157

Abstract excerpt

Shiga toxigenic Escherichia coli (STEC) comprises a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, certain strains appear to be of greater virulence for humans, for example, those belonging to serogroups O111 and O157 and those with particular combinations of other putative virulence traits. We have developed two multiplex PCR assays for the...

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