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Development of Monoclonal Antibodies and Immunoassays for Sensitive and Specific Detection of Shiga Toxin Stx2f

17 Sept 2013

Abstract excerpt

BACKGROUND: Shiga toxin 2 (Stx2) is a major virulence factor in gastrointestinal diseases caused by Escherichia coli. Although Stx2a (prototypical Stx2) is well-studied, all seven subtypes of Stx2 have been associated with disease in mammals. Several subtypes of Stx2, including Stx2f, are difficult to detect immunologically. METHODS AND FINDINGS: Four novel monoclonal antibodies (mAbs) against the Stx2f subtype...

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PMID: 24069462
DOI: 10.1371/journal.pone.0076563

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