Article
Comparison of 14 PCR systems for the detection and subtyping of stx genes in Shiga-toxin-producing Escherichia coli.
Research in microbiology - 1 Jan 2000
Bastian S N, Carle I, Grimont F
Abstract excerpt
The specificity of 14 polymerase chain reaction (PCR) systems designed for the detection and subtyping of stx genes was tested on a set of Escherichia coli strains with known sequences of stx genes. Systems designed for the detection of genes of the stx1 type did not detect any variant genes of t...
Topics
- Bacterial Toxins
- DNA Primers
- DNA Restriction Enzymes
- DNA, Bacterial
- Electrophoresis, Agar Gel
- Enterotoxins
- Escherichia coli
- Genetic Variation
- Phylogeny
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Shiga Toxin 1
- Shiga Toxin 2
- Virulence