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Detection of mutations by flow cytometric melting point analysis of PCR products.

Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Sept 2011

Imre László, Balogh István, Kappelmayer János, Szabó Miklós, Melegh Béla, Wanker Erich, Szabó Gábor

Abstract excerpt

Exploring the possibilities offered by flow cytometric microbead analyses for the detection of genetic alterations, an assay based on the dependence of the melting point of double-stranded DNA molecules on their length has been developed, making use of PCR products carrying biotin and fluorescent moiety on their two ends. The samples of different length PCR products immobilized on streptavidine coated microbeads...

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Identifiers

PMID: 21774077
DOI: 10.1002/cyto.a.21104

Genes

BRCA1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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