Article
Determination of melting temperature for variant detection using dHPLC: a comparison between an empirical approach and DNA melting prediction software.
Genetic testing - 1 Jan 2002
Rudolph Joseph G, White Stephanie, Sokolsky Cara, Bozak David, Mazzanti Chiarra, Lipsky Robert H, Goldman David
Abstract excerpt
Detection of DNA sequence variants by the use of denaturing high-performance liquid chromatography (dHPLC) is a relatively new method (Underhill et al., 1997) and has distinct advantages over other methods such as single-strand conformation polymorphism (SSCP), direct sequencing, and DNA chip hybridization. The dHPLC-based single-nucleotide polymorphism (SNP) screening relies on different DNA thermodynamic...
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