Article
Combination of multiplex PCR and DHPLC-based strategy for CYP2D6 genotyping scheme in Thais.
Clinical biochemistry - 1 Sept 2011
Suwannasri Payiarat, Thongnoppakhun Wanna, Pramyothin Pornpen, Assawamakin Anunchai, Limwongse Chanin
Abstract excerpt
OBJECTIVE: To develop CYP2D6 genotyping scheme for accurate allele calling and reliable estimation of functional allele dosage in Thais. DESIGN AND METHODS: We analyzed CYP2D6 copy numbers by pentaplex PCR coupled with semi-quantitative denaturing high performance liquid chromatography (DHPLC)-based technique. Ten common SNPs were genotyped from CYP2D6 gene product using single base extension (SBE) followed by...
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