Article
Robust CYP2D6 genotype assay including copy number variation using multiplex single-base extension for Asian populations.
Clinica chimica acta; international journal of clinical chemistry - 14 Dec 2010
Kim Eun-Young, Lee Sang-Seop, Jung Hyun-Ju, Jung Hye-Eun, Yeo Chang-Woo, Shon Ji-Hong, Shin Jae-Gook
Abstract excerpt
BACKGROUND: We developed a CYP2D6 genotyping method that includes copy number variation (CNV) and recently known functional haplotypes using multiplex single-base extension (SBE). METHODS: Twelve CYP2D6 alleles (*1, *2, *5, *10, *14, *18, *21, *41, *49, *52, *60, and a duplication of CYP2D6) were genotyped using 2 PCR reactions followed by multiplex SBE with 10 primers and singleplex SBE with 1 primer. The result...
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