Article
Fluorescence correlation spectroscopy investigation of a GFP mutant-enhanced cyan fluorescent protein and its tubulin fusion in living cells with two-photon excitation.
Journal of biomedical optics - 1 Jan 2000
Wang Zifu, Shah Jagesh V, Chen Zhongping, Sun Chung-Ho, Berns Michael W
Abstract excerpt
This study investigates the feasibility of using the enhanced cyan mutant of green fluorescent protein (ECFP) as a probe for two-photon fluorescence correlation spectroscopy (FCS). Molecular dynamics and other properties of ECFP and an ECFP-tubulin fusion protein were investigated in living Potor...
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