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Article

Mechanism and bottlenecks in strand photodissociation of split green fluorescent proteins (GFPs).

Proceedings of the National Academy of Sciences of the United States of America - 14 Mar 2017

Lin Chi-Yun, Both Johan, Do Keunbong, Boxer Steven G

Abstract excerpt

Split GFPs have been widely applied for monitoring protein-protein interactions by expressing GFPs as two or more constituent parts linked to separate proteins that only fluoresce on complementing with one another. Although this complementation is typically irreversible, it has been shown previously that light accelerates dissociation of a noncovalently attached β-strand from a circularly permuted split GFP,...

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Identifiers

PMID: 28242710
DOI: 10.1073/pnas.1618087114
PMCID: PMC5358378

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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