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Ultrafast photoconversion of the green fluorescent protein studied by accumulative femtosecond spectroscopy.

Biophysical journal - 8 Apr 2009

Langhojer Florian, Dimler Frank, Jung Gregor, Brixner Tobias

Abstract excerpt

The irreversible photoconversion of T203V green fluorescent protein (GFP) via decarboxylation is studied under femtosecond excitation using an accumulative product detection method that allows us to measure small conversion efficiencies of down to DeltaOD = 10(-7) absorbance change per pulse. Power studies with 800- and 400-nm pulse excitation reveal that excitation to higher states of the neutral form of the GFP...

Topics

Color
Fluorescence
Green Fluorescent Proteins
Kinetics
Mutation
Photochemical Processes
Spectrum Analysis
Time Factors

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 19348759
DOI: 10.1016/j.bpj.2008.11.049
PMCID: PMC2711285

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