Regulation of phosphoenolpyruvate carboxykinase gene expression by insulin. Use of the stable transfection approach to locate an insulin responsive sequence.

doi:10.1210/mend-4-9-1302

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Article

Regulation of phosphoenolpyruvate carboxykinase gene expression by insulin. Use of the stable transfection approach to locate an insulin responsive sequence.

Molecular endocrinology (Baltimore, Md.) - 1 Sept 1990

Forest C D, O'Brien R M, Lucas P C, Magnuson M A, Granner D K

Abstract excerpt

H4IIE rat hepatoma cells were stably transfected with various phosphoenolpyruvate carboxykinase-chloramphenicol acetyltransferase (PEPCK-CAT) expression vectors. The regulation of the transfected genes was qualitatively similar to that of the endogenous PEPCK gene. CAT expression was increased in response to cAMP and dexamethasone and insulin overrode these effects at concentrations known to be effective in...

Topics

Animals
Base Sequence
Chloramphenicol O-Acetyltransferase
Cyclic AMP
Deoxyribonuclease BamHI
Deoxyribonuclease HindIII
Dexamethasone
Gene Expression Regulation
Insulin
Liver Neoplasms, Experimental

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Phosphoenolpyruvate Carboxykinase (GTP)

Plasmids

Promoter Regions, Genetic

RNA, Messenger

Rats

Transfection

Source

Open in PubMed

Identifiers

PMID: 2172798
DOI: 10.1210/mend-4-9-1302

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2172798-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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