Article
Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II.
Applied and environmental microbiology - 1 Apr 1991
Ohta K, Beall D S, Mejia J P, Shanmugam K T, Ingram L O
Abstract excerpt
Zymomonas mobilis genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pfl). Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was...
Topics
- Acetyltransferases
- Alcohol Dehydrogenase
- Chloramphenicol Resistance
- Chromosomes, Bacterial
- Cloning, Molecular
- Escherichia coli
- Ethanol
- Fermentation
- Gene Amplification
- Genes, Bacterial
- Gram-Negative Bacteria
- Hydrogen
- Kinetics
- Mutation