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Article

Efficient immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases.

PloS one - 1 Jan 2011

Flisikowska Tatiana, Thorey Irmgard S, Offner Sonja, Ros Francesca, Lifke Valeria, Zeitler Bryan, Rottmann Oswald, Vincent Anna, Zhang Lei, Jenkins Shirin, Niersbach Helmut, Kind Alexander J, Gregory Philip D, Schnieke Angelika E, Platzer Josef

Abstract excerpt

Rabbits are widely used in biomedical research, yet techniques for their precise genetic modification are lacking. We demonstrate that zinc finger nucleases (ZFNs) introduced into fertilized oocytes can inactivate a chosen gene by mutagenesis and also mediate precise homologous recombination with a DNA gene-targeting vector to achieve the first gene knockout and targeted sequence replacement in rabbits. Two ZFN...

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Identifiers

PMID: 21695153
DOI: 10.1371/journal.pone.0021045
PMCID: PMC3113902

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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