Purification methods for recombinant Lactobacillus casei thymidylate synthase and mutants: a general, automated procedure.

doi:10.1016/s1046-5928(05)80039-7

Back to search

Article

Purification methods for recombinant Lactobacillus casei thymidylate synthase and mutants: a general, automated procedure.

Protein expression and purification - 1 Oct 1992

Kealey J T, Santi D V

Abstract excerpt

General procedures for the rapid, large-scale purification of recombinant Lactobacillus casei thymidylate synthase and its mutants have been established. An effective method employs sequential phosphocellulose and hydroxylapatite chromatography. Crude cell extracts are directly applied to phospho...

Topics

Bacterial Proteins
Cellulose
Chromatography, Ion Exchange
Durapatite
Hydroxyapatites
Lacticaseibacillus casei
Mutation
Recombinant Proteins
Thymidylate Synthase

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.