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Article

Purification methods for recombinant Lactobacillus casei thymidylate synthase and mutants: a general, automated procedure.

Protein expression and purification - 1 Oct 1992

Kealey J T, Santi D V

Abstract excerpt

General procedures for the rapid, large-scale purification of recombinant Lactobacillus casei thymidylate synthase and its mutants have been established. An effective method employs sequential phosphocellulose and hydroxylapatite chromatography. Crude cell extracts are directly applied to phosphocellulose, and the enzyme is obtained in a nearly pure state by stepwise elution with KCl. The eluate is directly...

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PMID: 1333860
DOI: 10.1016/s1046-5928(05)80039-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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